ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32795389.
- Also identified by DOI 10.7554/eLife.58573 and PMC identifier 7442491.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Alkb homolog 7 (ALKBH7) is a mitochondrial α-ketoglutarate dioxygenase required for DNA alkylation-induced necrosis, but its function and substrates remain unclear. Herein, we show ALKBH7 regulates dialdehyde metabolism, which impacts the cardiac response to ischemia-reperfusion (IR) injury. Using a multi-omics approach, we find no evidence ALKBH7 functions as a prolyl-hydroxylase, but we do find <i>Alkbh7</i><sup>-/-</sup> mice have elevated glyoxalase I (GLO-1), a dialdehyde detoxifying enzyme. Metabolic pathways related to the glycolytic by-product methylglyoxal (MGO) are rewired in <i>Alkbh7</i><sup>-/-</sup> mice, along with elevated levels of MGO protein adducts. Despite greater glycative stress, hearts from <i>Alkbh7</i><sup>-/-</sup> mice are protected against IR injury, in a manner blocked by GLO-1 inhibition. Integrating these observations, we propose ALKBH7 regulates glyoxal metabolism, and that protection against necrosis and cardiac IR injury bought on by ALKBH7 deficiency originates from the signaling response to elevated MGO stress.
Medical subject headings
- AlkB Enzymes
- Glyoxal
- Metabolic Networks and Pathways
- Necrosis
- Reperfusion Injury