Genotoxic effect of 2,2'-bis(bicyclo[2.2.1] heptane) on bacterial cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32822344.
- Also identified by DOI 10.1371/journal.pone.0228525 and PMC identifier 7444485.
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Abstract
The toxic effect of strained hydrocarbon 2,2'-bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E. coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.
Medical subject headings
- Bridged Bicyclo Compounds
- Escherichia coli
- Mutagens