Two-color nanoscopy of organelles for extended times with HIDE probes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32848153.
- Also identified by DOI 10.1038/s41467-020-17859-1 and PMC identifier 7450044.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables two-color super-resolution (STED) and 3D confocal imaging of two organelles simultaneously for extended times using high-density environmentally sensitive (HIDE) probes. Because HIDE probes are small, cell-permeant molecules, they can visualize dual organelle dynamics in hard-to-transfect cell lines by super-resolution for over an order of magnitude longer than with tagged proteins. The extended time domain possible using these tools reveals dynamic nanoscale targeting between different organelles.
Medical subject headings
- Fluorescent Dyes
- Microscopy, Fluorescence
- Nanotechnology
- Organelles