Fast and multiplexed superresolution imaging with DNA-PAINT-ERS.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32859909.
- Also identified by DOI 10.1038/s41467-020-18181-6 and PMC identifier 7455722.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.
Medical subject headings
- Cytological Techniques
- DNA
- Microscopy, Fluorescence
- Molecular Docking Simulation