Lesson of the month: novel method to quantify neutrophil uptake in early lung cancer using SPECT-CT.

Farahi, Neda; Gillett, Daniel; Southwood, Mark; Rassl, Doris; Tregay, Nicola; Hill, Uta; Preston, Stephen Denis; Loutsios, Chrystalla et al. · Thorax · 2020

basic_science · Level V

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Abstract

Neutrophils play an important role in the lung tumour microenvironment. We hypothesised that radiolabelled neutrophils coupled to single-photon emission CT (SPECT) may non-invasively quantify neutrophil uptake in tumours from patients with non-small cell lung cancer. We demonstrated increased uptake of radiolabelled neutrophils from the blood into tumours compared with non-specific uptake using radiolabelled transferrin. Moreover, indium-111-neutrophil activity in the tumour biopsies also correlated with myeloperoxidase (MPO)-positive neutrophils. Our data support the utility of imaging with In-111-labelled neutrophils and SPECT-CT to quantify neutrophil uptake in lung cancer.

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