Preparation and characterisation of nanofibrous cellulose plate as a new solid support for microbial culture.

Deguchi, Shigeru; Tsudome, Mikiko; Shen, Yihong; Konishi, Satoshi; Tsujii, Kaoru; Ito, Susumu; Horikoshi, Koki · Soft Matter · 2007

basic_science · Level V

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Abstract

Porous plates made of nanofibrous crystalline cellulose were prepared, and used as a solid support for microbial cultures. Representative mesophilic microorganisms (, , and ) grew on the cellulose plate, just as well as they did on the conventional agar plate. optical microscopic examination revealed that the cellulose plate remained unchanged up to 280 °C at a constant pressure of 25 MPa. Due to the structural stability at high temperatures, a representative thermophile, , was cultured successfully on the cellulose plate at 80 °C. Mouse fibroblast cells did not show significant adhesion or extension on the cellulose plate.