Theory of fluorescence correlation spectroscopy at variable observation area for two-dimensional diffusion on a meshgrid.
basic_science · Level V
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- Record sourced from PubMed, PMID 32907274.
- Also identified by DOI 10.1039/b718583a.
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Abstract
It has recently been proposed, with the help of numerical investigations, that fluorescence correlation spectroscopy at variable observation area can reveal the existence of a meshgrid of semi-permeable barriers hindering the two-dimensional diffusion of tagged particles, such as plasmic membrane constituents. We present a complete theory confirming and accounting for these findings. It enables a reliable, quantitative exploitation of experimental data from which the sub-wavelength mesh size can be extracted. Time-scales at which fluorescence correlation spectroscopy must be performed experimentally are discussed in detail.