Time-restricted feeding alters lipid and amino acid metabolite rhythmicity without perturbing clock gene expression.
rct · Level II
Where this comes from
- Record sourced from PubMed, PMID 32938935.
- Also identified by DOI 10.1038/s41467-020-18412-w and PMC identifier 7495469.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Time-restricted feeding (TRF) improves metabolism independent of dietary macronutrient composition or energy restriction. To elucidate mechanisms underpinning the effects of short-term TRF, we investigated skeletal muscle and serum metabolic and transcriptomic profiles from 11 men with overweight/obesity after TRF (8 h day<sup>-1</sup>) and extended feeding (EXF, 15 h day<sup>-1</sup>) in a randomised cross-over design (trial registration: ACTRN12617000165381). Here we show that muscle core clock gene expression was similar after both interventions. TRF increases the amplitude of oscillating muscle transcripts, but not muscle or serum metabolites. In muscle, TRF induces rhythmicity of several amino acid transporter genes and metabolites. In serum, lipids are the largest class of periodic metabolites, while the majority of phase-shifted metabolites are amino acid related. In conclusion, short-term TRF in overweight men affects the rhythmicity of serum and muscle metabolites and regulates the rhythmicity of genes controlling amino acid transport, without perturbing core clock gene expression.
Medical subject headings
- Amino Acids
- Circadian Rhythm
- Fasting
- Lipids
- Muscle, Skeletal