A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33009392.
- Also identified by DOI 10.1038/s41467-020-18744-7 and PMC identifier 7532196.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.
Medical subject headings
- Bacterial Proteins
- DNA, Bacterial
- Pseudomonas syringae
- Transcription Factors