An efficient KRAB domain for CRISPRi applications in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33020655.
- Also identified by DOI 10.1038/s41592-020-0966-x.
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Abstract
Clustered regularly interspaced short palindromic repeat interference (CRISPRi), based on the fusion of inactive Cas9 (dCas9) to the Krüppel-associated box (KRAB) repressor, is a powerful platform for silencing gene expression. However, it suffers from incomplete silencing of target genes. We assayed 57 KRAB domains for their repressive potency and identified the ZIM3 KRAB domain as an exceptionally potent repressor. We establish that ZIM3 KRAB-dCas9 fusion silences gene expression more efficiently than existing platforms.
Medical subject headings
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Kruppel-Like Transcription Factors