CRISPRoff enables spatio-temporal control of CRISPR editing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33028827.
- Also identified by DOI 10.1038/s41467-020-18853-3 and PMC identifier 7542431.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Light
- RNA Stability
- RNA, Guide, CRISPR-Cas Systems