Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer.
case_series · Level IV
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- Record sourced from PubMed, PMID 33105193.
- Also identified by DOI 10.14309/ajg.0000000000000939.
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Abstract
Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers. Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0-4 vs 4-8 vs 8-15 minutes). Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible. The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.
Medical subject headings
- Biomarkers
- Biomarkers/metabolism
- Carcinoma, Pancreatic Ductal
- Carcinoma, Pancreatic Ductal/diagnosis
- Carcinoma, Pancreatic Ductal/metabolism
- Early Detection of Cancer
- Early Detection of Cancer/methods
- Endosonography
- Humans
- Pancreatic Juice
- Pancreatic Juice/metabolism
- Pancreatic Neoplasms
- Pancreatic Neoplasms/diagnosis
- Pancreatic Neoplasms/metabolism
- Prospective Studies