O-Pair Search with MetaMorpheus for O-glycopeptide characterization.
basic_science · Level V
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- Record sourced from PubMed, PMID 33106676.
- Also identified by DOI 10.1038/s41592-020-00985-5 and PMC identifier 7606753.
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Abstract
We report O-Pair Search, an approach to identify O-glycopeptides and localize O-glycosites. Using paired collision- and electron-based dissociation spectra, O-Pair Search identifies O-glycopeptides via an ion-indexed open modification search and localizes O-glycosites using graph theory and probability-based localization. O-Pair Search reduces search times more than 2,000-fold compared to current O-glycopeptide processing software, while defining O-glycosite localization confidence levels and generating more O-glycopeptide identifications. Beyond the mucin-type O-glycopeptides discussed here, O-Pair Search also accepts user-defined glycan databases, making it compatible with many types of O-glycosylation. O-Pair Search is freely available within the open-source MetaMorpheus platform at https://github.com/smith-chem-wisc/MetaMorpheus .
Medical subject headings
- Glycopeptides
- Proteomics
- Tandem Mass Spectrometry