CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 33157047.
- Also identified by DOI 10.1016/j.stem.2020.10.014.
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Abstract
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Organoids