The smfBox is an open-source platform for single-molecule FRET.
basic_science · Level V
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- Record sourced from PubMed, PMID 33159061.
- Also identified by DOI 10.1038/s41467-020-19468-4 and PMC identifier 7648814.
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Abstract
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.