Urine metabolomics of rats with chronic atrophic gastritis.

Zu, Guo-Xiu; Sun, Qian-Qian; Chen, Jian; Liu, Xi-Jian; Sun, Ke-Yun; Zhang, Liang-Kun; Li, Ling; Han, Tao et al. · PLoS One · 2020

basic_science · Level V

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Abstract

To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG). Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method. Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism. By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.

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