Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33177502.
- Also identified by DOI 10.1038/s41467-020-19510-5 and PMC identifier 7659323.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB<sub>1</sub>), we discovered periodically repeating clusters of CB<sub>1</sub> hotspots within the axons of neurons. We observed these CB<sub>1</sub> hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB<sub>1</sub> signaling. Furthermore, we found that CB<sub>1</sub> hotspot periodicity increased upon CB<sub>1</sub> agonist application, and these activated CB<sub>1</sub> displayed less dynamic movement compared to non-activated CB<sub>1</sub>. Our results suggest that CB<sub>1</sub> forms periodic hotspots organized by the MPS as a mechanism to increase signaling efficacy upon activation.
Medical subject headings
- Brain
- Molecular Imaging
- Neurons
- Receptor, Cannabinoid, CB1