Plasma microRNA signature in presymptomatic and symptomatic subjects with <i>C9orf72</i>-associated frontotemporal dementia and amyotrophic lateral sclerosis.

Abstract

To identify potential biomarkers of preclinical and clinical progression in chromosome 9 open reading frame 72 gene (<i>C9orf72)</i>-associated disease by assessing the expression levels of plasma microRNAs (miRNAs) in <i>C9orf72</i> patients and presymptomatic carriers. The PREV-DEMALS study is a prospective study including 22 <i>C9orf72</i> patients, 45 presymptomatic <i>C9orf72</i> mutation carriers and 43 controls. We assessed the expression levels of 2576 miRNAs, among which 589 were above noise level, in plasma samples of all participants using RNA sequencing. The expression levels of the differentially expressed miRNAs between patients, presymptomatic carriers and controls were further used to build logistic regression classifiers. Four miRNAs were differentially expressed between patients and controls: miR-34a-5p and miR-345-5p were overexpressed, while miR-200c-3p and miR-10a-3p were underexpressed in patients. MiR-34a-5p was also overexpressed in presymptomatic carriers compared with healthy controls, suggesting that miR-34a-5p expression is deregulated in cases with <i>C9orf72</i> mutation. Moreover, miR-345-5p was also overexpressed in patients compared with presymptomatic carriers, which supports the correlation of miR-345-5p expression with the progression of <i>C9orf72</i>-associated disease. Together, miR-200c-3p and miR-10a-3p underexpression might be associated with full-blown disease. Four presymptomatic subjects in transitional/prodromal stage, close to the disease conversion, exhibited a stronger similarity with the expression levels of patients. We identified a signature of four miRNAs differentially expressed in plasma between clinical conditions that have potential to represent progression biomarkers for <i>C9orf72</i>-associated frontotemporal dementia and amyotrophic lateral sclerosis. This study suggests that dysregulation of miRNAs is dynamically altered throughout neurodegenerative diseases progression, and can be detectable even long before clinical onset. NCT02590276.