Vitamin D metabolites and the gut microbiome in older men.

Thomas, Robert L; Jiang, Lingjing; Adams, John S; Xu, Zhenjiang Zech; Shen, Jian; Janssen, Stefan; Ackermann, Gail; Vanderschueren, Dirk et al. · Nat Commun · 2020

cross_sectional · Level IV

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Abstract

The vitamin D receptor is highly expressed in the gastrointestinal tract where it transacts gene expression. With current limited understanding of the interactions between the gut microbiome and vitamin D, we conduct a cross-sectional analysis of 567 older men quantifying serum vitamin D metabolites using LC-MSMS and defining stool sub-Operational Taxonomic Units from16S ribosomal RNA gene sequencing data. Faith's Phylogenetic Diversity and non-redundant covariate analyses reveal that the serum 1,25(OH)<sub>2</sub>D level explains 5% of variance in α-diversity. In β-diversity analyses using unweighted UniFrac, 1,25(OH)<sub>2</sub>D is the strongest factor assessed, explaining 2% of variance. Random forest analyses identify 12 taxa, 11 in the phylum Firmicutes, eight of which are positively associated with either 1,25(OH)<sub>2</sub>D and/or the hormone-to-prohormone [1,25(OH)<sub>2</sub>D/25(OH)D] "activation ratio." Men with higher levels of 1,25(OH)<sub>2</sub>D and higher activation ratios, but not 25(OH)D itself, are more likely to possess butyrate producing bacteria that are associated with better gut microbial health.

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