Cas9 in Human Embryos: On Target but No Repair.
editorial · Level V
Where this comes from
- Record sourced from PubMed, PMID 33306952.
- Also identified by DOI 10.1016/j.cell.2020.11.022.
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Abstract
In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for "gene surgery".
Medical subject headings
- DNA Breaks, Double-Stranded
- DNA End-Joining Repair