Convergence of 3D printed biomimetic wound dressings and adult stem cell therapy.

Shafiee, Abbas; Cavalcanti, Amanda S; Saidy, Navid T; Schneidereit, Dominik; Friedrich, Oliver; Ravichandran, Akhilandeshwari; De-Juan-Pardo, Elena M; Hutmacher, Dietmar W · Biomaterials · 2021

basic_science · Level V

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Abstract

Biomimetically designed medical-grade polycaprolactone (mPCL) dressings are 3D-printed with pore architecture and anisotropic mechanical characteristics that favor skin wound healing with reduced scarring. Melt electrowritten mPCL dressings are seeded with human gingival tissue multipotent mesenchymal stem/stromal cells and cryopreserved using a clinically approved method. The regenerative potential of fresh or frozen cell-seeded mPCL dressing is compared in a splinted full-thickness excisional wound in a rat model over six weeks. The application of 3D-printed mPCL dressings decreased wound contracture and significantly improved skin regeneration through granulation and re-epithelialization compared to control groups. Combining 3D-printed biomimetic wound dressings and precursor cell delivery enhances physiological wound closure with reduced scar tissue formation.

Medical subject headings