Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33321074.
- Also identified by DOI 10.1016/j.stem.2020.11.014 and PMC identifier 7837212.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.
Medical subject headings
- Neoplasms
- Pluripotent Stem Cells