Conformation of the nuclear pore in living cells is modulated by transport state.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33346731.
- Also identified by DOI 10.7554/eLife.60654 and PMC identifier 7752133.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
While the static structure of the nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, <i>in vivo</i> conformational changes of the NPC remain under-explored. We developed sensors that report on the orientation of NPC components by rigidly conjugating mEGFP to different NPC proteins. Our studies show conformational changes to select domains of nucleoporins (Nups) within the inner ring (Nup54, Nup58, Nup62) when transport through the NPC is perturbed and no conformational changes to Nups elsewhere in the NPC. Our results suggest that select components of the NPC are flexible and undergo conformational changes upon engaging with cargo.
Medical subject headings
- Active Transport, Cell Nucleus
- Nuclear Pore