Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33408404.
- Also identified by DOI 10.1038/s41592-020-01024-z.
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Abstract
We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm<sup>3</sup> at a lateral resolution of ~1 μm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.
Medical subject headings
- Brain
- Microscopy, Fluorescence, Multiphoton
- Miniaturization
- Neuroimaging