The validity and reliability of the Achilles tendon moment arm assessed with dual-energy X-ray absorptiometry, relative to MRI and ultrasound assessments.

Abstract

Dual-energy X-ray absorptiometry (DXA) in single energy mode has been shown to permit the visualisation of bone and soft tissue, such as the patellar tendon through two-dimensional sagittal imaging. However, there is no validated DXA-based measurement of the Achilles tendon moment arm (d_AT). The aims of this study were: 1) to compare in vivo DXA derived measurements of the d_AT at rest against two previously validated methods: tendon excursion (TE) and magnetic resonance imaging (MRI) at three ankle angles (-5°, 0° and +10°). 2) analyse the intra-day reliability of the DXA method at all ankle angles and compare between methods. Twelve healthy adults (mean ± SD: 31.4 ± 9.5 years; 174.0 ± 9.5 cm; 76.2 ± 16.6 kg) participated in this study, involving test-retest DXA scans, ultrasound scans and one MRI scan. The d_AT was defined as the distance from the centre of the calcaneal-tibial joint axis to the Achilles tendon (AT) muscle-tendon line of action. DXA derived d_AT measures were significantly greater than MRI measurements (19.7-24.9%) and were 45.2% significantly larger than the TE method. The test-retest reliability of the DXA technique at 0° was high [CV = 1.38%; ICC = 0.96] and despite the consistently larger d_AT lengths obtained using DXA, MRI and DEXA data were strongly correlated (r = 0.878, p < 0.001). In conclusion, the DXA technique allowed for highly reproducible in vivo d_AT measurement at rest, which has implications for the calculation of AT forces in vivo and the ability to predict the measurement from one tool to the other, thereby providing a novel basis to contrast existing and future studies.