Random access parallel microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33432922.
- Also identified by DOI 10.7554/eLife.56426 and PMC identifier 7843131.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and <i>Caenorhabditis elegans</i> preparations.
Medical subject headings
- Caenorhabditis elegans
- Microscopy