STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33473127.
- Also identified by DOI 10.1038/s41467-020-20826-5 and PMC identifier 7817837.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.
Medical subject headings
- Calcium
- Cell Membrane
- Cytoplasm
- ORAI1 Protein
- Stromal Interaction Molecule 1