Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33479249.
- Also identified by DOI 10.1038/s41467-020-20686-z and PMC identifier 7820506.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.
Medical subject headings
- DNA
- Microscopy, Fluorescence
- Nanostructures
- Nanotechnology