A three-dimensional in vitro culture environment of a novel polymer scaffold, yielding chondroprogenitors and mesenchymal stem cells in human chondrocytes derived from osteoarthritis-affected cartilage tissue.
basic_science · Level V
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- Record sourced from PubMed, PMID 33510554.
- Also identified by DOI 10.1016/j.jor.2021.01.005 and PMC identifier 7815488.
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Abstract
We evaluated the expression of stem/progenitor biomarkers in osteoarthritic tissue derived chondrocytes cultured using a three-dimensional (3D) thermo-reversible gelation polymer (TGP). The chondrocytes from discarded biopsy tissues obtained from human elderly patients with osteoarthritis were cultured using the 3D-TGP up to six weeks. The chondrocytes grew in a tissue-like manner, without de-differentiation into fibroblasts, and the cells thus tissue-engineered were proven positive for CD49e, OCT4, CD-105 and STRO-1 by immunohistochemistry. This study establishes the efficacy of this 3D-TGP platform for clinically useable <i>in-vitro</i> tissue-engineered cartilage for improvising the clinical outcome of cell therapy for cartilage repair.