Improved hemostatic effects by Fe³⁺ modified biomimetic PLLA cotton-like mat via sodium alginate grafted with dopamine.

Abstract

The development of an excellent, bioabsorbable hemostatic material for deep wound remains a challenge. In this work, a biodegradable cotton-like biomimetic fibrous mat of poly (l-lactic acid) (PLLA) was made by melt spinning. Subsequently, SD composite was prepared by cross-linking sodium alginate (SA) with dopamine (DA). It was immobilized on the fibre surface, which inspired by mussel byssus. Finally, Fe³⁺ was loaded onto the 0.5SD/PLLA composite by chelation with the carboxyl of alginate and phenolic hydroxy of dopamine. The haemostasis experiment found that the hemostatic time 47 s in vitro. However, the bleeding volume was 0.097 g and hemostatic time was 23 s when 20Fe³⁺-0.5SD/PLLA was applied in the haemostasis of the rat liver. As a result of its robust hydrophilicity and bouffant cotton-like structure, it could absorb a large water from blood, which could concentrate the component of blood and reduce the clotting time. Furthermore, the addition of Fe³⁺ in the 0.5SD/PLLA had a significant effect on improve hemostatic property. It also displayed excellent antibacterial property for <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Notably, it possesses superior hemocompatibility, cytocompatibility and histocompatibility. Hence, 20Fe³⁺-0.5SD/PLLA has high potential application in haemostasis for clinical settings due to its outstanding properties.