Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33574610.
- Also identified by DOI 10.1038/s41587-020-00794-3.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization microscopy with a resolution not limited by photobleaching. We use RhoBAST to image subcellular structures of RNA in live and fixed cells with about 10-nm localization precision and a high signal-to-noise ratio.
Medical subject headings
- Aptamers, Nucleotide
- Rhodamines