Decoding the protein composition of whole nucleosomes with Nuc-MS.
basic_science · Level V
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- Record sourced from PubMed, PMID 33589837.
- Also identified by DOI 10.1038/s41592-020-01052-9 and PMC identifier 7954958.
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Abstract
Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the 'histone code'. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.
Medical subject headings
- Histones
- Nucleosomes
- Proteomics
- Spectrometry, Mass, Electrospray Ionization