Genome-scale deconvolution of RNA structure ensembles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33619392.
- Also identified by DOI 10.1038/s41592-021-01075-w.
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Abstract
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
Medical subject headings
- Algorithms
- Genome, Viral
- Nucleic Acid Conformation
- RNA, Viral
- SARS-CoV-2