Highly multiplexed 2-dimensional imaging mass cytometry analysis of HBV-infected liver.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33621209.
- Also identified by DOI 10.1172/jci.insight.146883 and PMC identifier 8119221.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Studies of human hepatitis B virus (HBV) immune pathogenesis are hampered by limited access to liver tissues and technologies for detailed analyses. Here, utilizing imaging mass cytometry (IMC) to simultaneously detect 30 immune, viral, and structural markers in liver biopsies from patients with hepatitis B e antigen+ (HBeAg+) chronic hepatitis B, we provide potentially novel comprehensive visualization, quantitation, and phenotypic characterizations of hepatic adaptive and innate immune subsets that correlated with hepatocellular injury, histological fibrosis, and age. We further show marked correlations between adaptive and innate immune cell frequencies and phenotype, highlighting complex immune interactions within the hepatic microenvironment with relevance to HBV pathogenesis.
Medical subject headings
- Hepatitis B, Chronic
- Image Cytometry
- Liver