Shulin packages axonemal outer dynein arms for ciliary targeting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33632841.
- Also identified by DOI 10.1126/science.abe0526 and PMC identifier 7116892.
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Abstract
The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate <i>Tetrahymena</i> to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
Medical subject headings
- Axonemal Dyneins
- Cilia
- Protozoan Proteins
- Tetrahymena thermophila