A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33633410.
- Also identified by DOI 10.1038/s41592-021-01078-7.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Serotonin (5-HT) is an important signaling monoamine and neurotransmitter. We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick Argas monolakensis. G-GESS shows fast response kinetics and high affinity, specificity, brightness and photostability. We used G-GESS to image 5-HT dynamics in cultured cells, brain slices and behaving mice.
Medical subject headings
- Biosensing Techniques
- Lipocalins
- Optical Imaging
- Serotonin