Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33658510.
- Also identified by DOI 10.1038/s41467-021-21686-3 and PMC identifier 7930113.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after H<sub>2</sub>O<sub>2</sub> treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.
Medical subject headings
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Cysteine