Single-cell measurement of plasmid copy number and promoter activity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33674569.
- Also identified by DOI 10.1038/s41467-021-21734-y and PMC identifier 7935883.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Medical subject headings
- Bacteria
- DNA Copy Number Variations
- Plasmids