Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33674580.
- Also identified by DOI 10.1038/s41467-021-21627-0 and PMC identifier 7935920.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.
Medical subject headings
- COVID-19
- COVID-19 Testing
- Colorimetry
- Molecular Diagnostic Techniques
- Nucleic Acid Amplification Techniques
- Nucleic Acid Hybridization
- Point-of-Care Testing
- SARS-CoV-2