Integrative transcription start site identification with iTiSS.

Jürges, Christopher S; Dölken, Lars; Erhard, Florian · Bioinformatics · 2021

basic_science · Level V

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Abstract

Many experimental approaches have been developed to identify transcription start sites (TSS) from genomic scale data. However, experiment specific biases lead to large numbers of false-positive calls. Here, we present our integrative approach iTiSS, which is an accurate and generic TSS caller for any TSS profiling experiment in eukaryotes, and substantially reduces the number of false positives by a joint analysis of several complementary datasets. iTiSS is platform independent and implemented in Java (v1.8) and is freely available at https://www.erhard-lab.de/software and https://github.com/erhard-lab/iTiSS. Supplementary data are available at Bioinformatics online.

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