High-efficiency prime editing with optimized, paired pegRNAs in plants.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33767395.
- Also identified by DOI 10.1038/s41587-021-00868-w.
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Abstract
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.
Medical subject headings
- Gene Editing
- Oryza
- RNA, Plant