Oxidation of trimethylamine to trimethylamine <i>N</i>-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage.

Abstract

High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. <i>Myroides profundi</i> D25^T, obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine <i>N</i>-oxide (TMAO) by a TMA monooxygenase, <i>Mp</i>Tmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-<i>Mp</i>Tmm pathway was further confirmed by introducing it into <i>Escherichia coli</i> and <i>Bacillus subtilis</i> Encoded TmaT-like and <i>Mp</i>Tmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and <i>Mp</i>Tmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.