Molecular-scale axial localization by repetitive optical selective exposure.
basic_science · Level V
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- Record sourced from PubMed, PMID 33795876.
- Also identified by DOI 10.1038/s41592-021-01099-2.
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Abstract
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy