Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33846317.
- Also identified by DOI 10.1038/s41467-021-22377-9 and PMC identifier 8041833.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.
Medical subject headings
- Microscopy, Atomic Force
- Microscopy, Fluorescence