MAUI (MBI Analysis User Interface)-An image processing pipeline for Multiplexed Mass Based Imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33872301.
- Also identified by DOI 10.1371/journal.pcbi.1008887 and PMC identifier 8084329.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Mass Based Imaging (MBI) technologies such as Multiplexed Ion Beam Imaging by time of flight (MIBI-TOF) and Imaging Mass Cytometry (IMC) allow for the simultaneous measurement of the expression levels of 40 or more proteins in biological tissue, providing insight into cellular phenotypes and organization in situ. Imaging artifacts, resulting from the sample, assay or instrumentation complicate downstream analyses and require correction by domain experts. Here, we present MBI Analysis User Interface (MAUI), a series of graphical user interfaces that facilitate this data pre-processing, including the removal of channel crosstalk, noise and antibody aggregates. Our software streamlines these steps and accelerates processing by enabling real-time and interactive parameter tuning across multiple images.
Medical subject headings
- Image Processing, Computer-Assisted
- Proteins
- Single-Cell Analysis
- User-Computer Interface