CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33927418.
- Also identified by DOI 10.1038/s41587-021-00901-y and PMC identifier 8553808.
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Abstract
Prime editors have been delivered using DNA or RNA vectors. Here we demonstrate prime editing with purified ribonucleoprotein complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate germline transmission. We also observed unintended insertions, deletions and prime editing guide RNA (pegRNA) scaffold incorporations. In HEK293T and primary human T cells, prime editing with purified ribonucleoprotein complexes introduced desired edits with frequencies of up to 21 and 7.5%, respectively.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Zebrafish