Naive stem cell blastocyst model captures human embryo lineage segregation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33957081.
- Also identified by DOI 10.1016/j.stem.2021.04.031 and PMC identifier 8189436.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.
Medical subject headings
- Blastocyst
- Embryo, Mammalian