TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 33986545.
- Also identified by DOI 10.1038/s41592-021-01142-2 and PMC identifier 9328942.
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Abstract
We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer-even full-length-receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5' 10x Genomics platforms.
Medical subject headings
- Algorithms
- Receptors, Immunologic
- Sequence Analysis, RNA
- Single-Cell Analysis