Short-lived intermediate in N₂O generation by P450 NO reductase captured by time-resolved IR spectroscopy and XFEL crystallography.

Abstract

Nitric oxide (NO) reductase from the fungus <i>Fusarium oxysporum</i> is a P450-type enzyme (P450nor) that catalyzes the reduction of NO to nitrous oxide (N₂O) in the global nitrogen cycle. In this enzymatic reaction, the heme-bound NO is activated by the direct hydride transfer from NADH to generate a short-lived intermediate ( <i><u>I</u></i> ), a key state to promote N-N bond formation and N-O bond cleavage. This study applied time-resolved (TR) techniques in conjunction with photolabile-caged NO to gain direct experimental results for the characterization of the coordination and electronic structures of <i><u>I</u></i> TR freeze-trap crystallography using an X-ray free electron laser (XFEL) reveals highly bent Fe-NO coordination in <i><u>I</u></i> , with an elongated Fe-NO bond length (Fe-NO = 1.91 Å, Fe-N-O = 138°) in the absence of NAD⁺ TR-infrared (IR) spectroscopy detects the formation of <i><u>I</u></i> with an N-O stretching frequency of 1,290 cm^-1 upon hydride transfer from NADH to the Fe³⁺-NO enzyme via the dissociation of NAD⁺ from a transient state, with an N-O stretching of 1,330 cm^-1 and a lifetime of ca. 16 ms. Quantum mechanics/molecular mechanics calculations, based on these crystallographic and IR spectroscopic results, demonstrate that the electronic structure of <i><u>I</u></i> is characterized by a singly protonated Fe³⁺-NHO^•- radical. The current findings provide conclusive evidence for the N₂O generation mechanism via a radical-radical coupling of the heme nitroxyl complex with the second NO molecule.