Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 34035309.
- Also identified by DOI 10.1038/s41467-021-23449-6 and PMC identifier 8149693.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.
Medical subject headings
- Imaging, Three-Dimensional
- Intravital Microscopy
- Lighting